CACO-2
ACC 169
Cell line:
CACO-2
DSMZ no.:
ACC 169
Species:
human (Homo sapiens)
Cell type:
colon adenocarcinoma
Origin:
established from the primary colon tumor (adenocarcinoma) of a 72-year-old man in 1974; cells are described to produce mucus
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
This cell line will only be distributed to non-profit institutions. Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
Medium:
80% MEM (with Earle's salts) + 20% h.i. FBS + 1x non-essential amino acids
Subculture:
cells grow slowly; seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:3 to 1:6 every 5-7 days using trypsin/EDTA or, if necessary, a cell scraper; after thawing cells might start slowly to take up growth; to avoid prolonged trypsinization time (>10 min) and decreased viability, split the culture before complete confluency
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 70-80 hours
Harvest:
about 5-8 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated during accessioning with BM-Cyclin (tiamulin & minocycline), thereafter negative in microbiologcal culture, RNA hybridization, and continuously in PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list