Mycoplasma:
contamination was initially eliminated with Ciprobay (ciprofloxacin), thereafter negative in DAPI, microbiological culture, RNA hybridization and repeatedly in PCR assays
Immunology:
CD2 +, CD3 -, CD4 (+), CD13 -, CD14 -, CD15 +, CD19 -, CD21 -, CD25 +, CD30 +, HLA-DR +;
image
Cytogenetics:
remarkable human hypodiploid cell line with large number of rearrangements involving all chromosomes except ch 10 - 36(35-38)<2n>X, der(Y), breakpoints at: 1p21, 1q11, 2p14, 2q37, 3q21, 3p14, 3q11, 3q25.2, 3q27, 4q25, 5q11, 5q35, 6q15/16, 7p14, 8q21.1, 8q23, 8q24.2, 9p11, 11q13, 12p12, 12q24.3, 13p11, 13q14, 14q21, 14q23/24.2, 15p11, 15q13, 17p11, 17q25, 18p11, 19p13, 19q13, 21q21, 22q33, Ycen, Yq12
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
