3T3
ACC 173
Cell line:
3T3
DSMZ no.:
ACC 173
Species:
murine (mouse, Swiss albino mouse) (Mus musculus)
Cell type:
fibroblasts
Origin:
established from disaggregated Swiss albino mouse embryos in 1962; cells are used for transfection studies with DNA viruses and as assay system for transformation studies; cells have a high sensitivity to contact inhibition
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90-95% Dulbecco's MEM + 5-10% h.i. FBS
Subculture:
split confluent culture at a ratio of up to 1:8 every 2 to 4 days using trypsin/EDTA; after trypsination cells might remain in aggregates that are difficult to disperse; upon reaching confluence cells stop to grow; cells grow fast at low titer; seed out at ca. 1-2 x 106 cells/175 cm2
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 40 hours
Harvest:
cell harvest of about 5-8 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypertriploid (near tetraploid sideline present) karyotype with 13% polyploidy - 68(58-73)<3n>
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list