MDBK
ACC 174
Cell line:
MDBK
DSMZ no.:
ACC 174
Species:
bovine (Bos taurus)
Cell type:
kidney cells
Origin:
cells were derived from a kidney of a normal adult steer in 1957; cells are described to be BVD Virus-free (bovine diarrhea virus)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
95% DMEM + 5% h.i. FBS
Subculture:
seed out 1-2 x 106 cells/80 cm2; optimal split ratio at 1:4 to 1:6 using trypsin/EDTA; cells grow within 2-3 days to confluence
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35 hours
Harvest:
cell harvest of about 5-10 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Bos taurus species.
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -, BVDV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list