HEPA 1-6
ACC 175
Cell line:
HEPA 1-6
DSMZ no.:
ACC 175
Species:
murine (mouse) (Mus musculus)
Cell type:
hepatoma
Origin:
cell line was derived from the BW7756 tumor that arose in a C57L mouse; cells were described in the literature to secrete several liver-specific products (e.g. albumin, alpha1-antitrypsin, alpha-fetoprotein, amylase); cells may grow in serum-free medium when supplemented with dexamethasone and selenium
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% DMEM (4.5 g/L glucose) + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2 or 175 cm2; split confluent culture 1:4 to 1:10 every 3-6 days using trypsin/EDTA; cells grow rather quickly
Incubation:
at 37 °C with 10% CO2
Doubling time:
about 30 hours
Harvest:
about 10-15 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypotetraploid karyotype with centric fusion and 6% polyploidy - flat-moded, 53-70<4n>
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list