PA-TU-8902
ACC 179
Cell line:
PA-TU-8902
DSMZ no.:
ACC 179
Species:
human (Homo sapiens)
Cell type:
pancreas adenocarcinoma
Origin:
established in 1985 from the primary ductal pancreatic adenocarcinoma (grade II) from a 44-year-old woman; reported as inducing metastasis in nude mice, to carry mutations in KRAS and TP53 and to secrete cytokines and proteinases
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% DMEM (4.5 g/L glucose) + 10% h.i. FBS + 2 mM L-glutamine
Subculture:
seed out at ca. 0.5-2 x 106 cells/80 cm2; split confluent culture 1:4 to 1:10 every 3-6 days using trypsin/EDTA
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 25-40 hours
Harvest:
cell harvest of 4-8 x 106 cells/80cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded pseudotetraploid karyotype - 91(83-94)<4n>XXX-X, +5, -6, -6, +7, -10, -10, +12, -13, -13, -14, -19, +20, -22, -22, +5mar, del(4)(q26)x2, der(5)t(5;14)(p11;q11), der(5)t(5;?)(q11;?), der(7)t(7;?)(p14;?), der(9)t(9;11)(p12;q11), der(14)t(?5;14)(?p11;p11)x2, del(17)(p12), der(17)t(17;?)(p11/12;?), der(18)t(18;?)(p12;?), der(19)t(19;?)(q13.1;?), ?i(21q)x3, der(22)t(22;?)(q13;?) - carries 19p13.1 recurrent tumor breakpoint - corresponds to vague published description
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list