2HX-2
ACC 183
Cell line:
2HX-2
DSMZ no.:
ACC 183
Species:
murine (mouse) (Mus musculus)
Cell type:
hybridoma (anti-adenovirus 2)
Origin:
established by fusion of SP2/0-AG14 myeloma cells with spleen cells from BALB/c mice immunized with HELA cells that were infected with adenovirus 2; cells secrete an IgG monoclonal antibody reacting with an adenovirus group specific antigen; cell line is patented
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
single cells growing in suspension
Medium:
90% Dulbecco's MEM (4.5 g/L glucose) + 10% h.i. FBS
Subculture:
optimal split ratio of 1:2 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Harvest:
cell harvest of about 1-2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine karyotype with centric fusion and 16% polyploidy - 66(58-74)<3n>
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list