EFO-27
ACC 191
Cell line:
EFO-27
DSMZ no.:
ACC 191
Species:
human (Homo sapiens)
Cell type:
ovary adenocarcinoma
Origin:
established from the solid omental metastasis of a mucinous papillary adenocarcinoma of the ovary of a 36-year-old woman in 1979; initially diploid, later tetraploid; growth in nude mice
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine + 1x MEM non-essential amino acids + 1 mM sodium pyruvate
Subculture:
seed out at ca. 0.5-1.5 x 106 cells/80 cm2; split confluent culture 1:2 to 1:5 every 2-5 days using trypsin/EDTA (incubate for 10-20 minutes with trypsin/EDTA);
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 50 hours
Harvest:
cell harvest of about 0.3-0.6 x 105 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypotetraploid karyotype with 17% polyploidy - 78-91<4n>XXXX, -4, -10, -13, -13, -19, +mar - i(9q), 16qh+x2 - extensive subclonal rearrangements, e.g. der(5)t(5;7)(q31.2;q11)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list