COLO-678
ACC 194
Cell line:
COLO-678
DSMZ no.:
ACC 194
Species:
human (Homo sapiens)
Cell type:
colon carcinoma
Origin:
established from a metastatic lymph node of a 69-year-old man with colon carcinoma in 1984; extensively characterized and frequently used cell line; part of the CCLE (ref 18232)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/175 cm2; split confluent culture 1:4 every 6-7 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60-80 hours
Harvest:
cell harvest of about 20-25 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 4% polyploidy - 57(52-58)<2n>XXY, +X, +3, +7, +8, +10, +11, +12, +15, -17, -18, +22, +4mar, add(3)(p11), amp(7)(q11.23)x2, del(12)(q21/22q24.2)x2 - trisomy 7 and 12 are associated with colon carcinoma
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list