3T6
ACC 202
Cell line:
3T6
DSMZ no.:
ACC 202
Species:
murine (mouse, Swiss albino mouse) (Mus musculus)
Cell type:
fibroblasts
Origin:
established from disaggregated Swiss albino mouse embryos in 1963; cells were described in the literature to secrete collagen and hyaluronic acid
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
85-90% DMEM + 10-15% h.i. FBS
Subculture:
split confluent culture ca. 1:10 every 2-3 days using trypsin/EDTA, seed out about 2 x 106 cells/175 cm2; cells may clump after trypsinization and may be difficult to disperse
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 15 hours
Harvest:
cell harvest of about 15 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine flat-moded hypertriploid/hypotetraploid karyotype - 67(64-73)<3n>
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list