2M6
ACC 205
Cell line:
2M6
DSMZ no.:
ACC 205
Species:
murine (mouse) (Mus musculus)
Cell type:
hybridoma (anti-chicken retina)
Origin:
cell line was established by fusion of mouse NS-1 cells with spleen from a BALB/c mouse immunized with Triton X114-purified chicken retina E10 proteins; described as secreting a mouse monoclonal antibody (IgG) reacting with chicken retina (Mueller glia cell-specific antigen)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80-85% RPMI 1640 + 10-15% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement") for the first 1-2 weeks post-thawing
Subculture:
cells are difficult to culture! maintain at ca. 0.2-0.8 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-3 days; seed out at ca. 0.2 x 106 cells/ml; after thawing cultivate cells for the first 1-2 weeks in 24- or 12-well-plates using HFCS
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35 hours
Harvest:
maximal density at ca. 0.8 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
secretion of IgG1 monoclonal antibody
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypertetraploid karyotype
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list