SH-SY5Y
ACC 209
Cell line:
SH-SY5Y
DSMZ no.:
ACC 209
Species:
human (Homo sapiens)
Cell type:
neuroblastoma
Origin:
clonal subline of the neuroepithelioma cell line SK-N-SH that had been established in 1970 from the bone marrow biopsy of a 4-year-old girl with metastatic neuroblastoma (supposedly N-type and described to carry a ALK mutation). Widely used and extensively characterized cell line; listed in the CCLE (ref 18232)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80-85% DMEM + 15-20% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells in a T75 flask; split culture 1-2 times a week using trypsin/EDTA; selection of neuronal-like cells may be favored by reduction of glutamine (1 mM), passaging of semi- or half-confluent cultures; short-time trypsin exposure preferentially detaches the epithelial-like cells
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 50-60 hours
Harvest:
cell harvest of about 15 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-diploid karyotype with 1.8% polyploidy - 46/47(42-48)<2n>X/XX, +7, ins(1)(q32q11q43), add(9)(q34), der(22)t(?17;22)(q22;q13) - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list