MC3T3-E1
ACC 210
Cell line:
MC3T3-E1
DSMZ no.:
ACC 210
Species:
murine (mouse) (Mus musculus)
Cell type:
embryo/fetus calvaria fibroblasts
Origin:
established from the calvaria of an embryo/fetus C57BL/6 mouse; described in the literature to differentiate to osteoblasts and to produce collagen
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10-20% h.i. FBS + 2 mM L-glutamine
Subculture:
seed out at about 1-2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 once or twice a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 1-2 days
Harvest:
cell harvest of ca. 6-7 x 106 cells/80 cm2 or 9 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypotetraploid karyotype with 11.5% polyploidy - 72(68-80)<4n> - centric fusion absent
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list