COLO-849

human (Homo sapiens)

melanoma

derived in 1992 from right axillary lymph nodes of a 43-year-old man with metastatic maligant melanoma

1

A

fibroblastic cells growing mainly as adherent monolayer and partly in suspension; sedimented cells have a brown color (suggesting production of melanin); image; image; image

80-90% RPMI 1640 + 10-20% h.i. FBS

split confluent culture 1:4 to 1:10 every 4-7 days using trypsin/EDTA; seed out at ca. 2-3 x 10⁶ cells/80 cm²

at 37 °C with 5% CO₂

ca. 50-60 hours

cell harvest of about 18 x 10⁶ cells/175 cm²

frozen with 70% medium, 20% FBS, 10% DMSO

negative in DAPI, microbiological culture, RNA hybridization, PCR assays

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

human hypotriploid karyotype - 60<3n>X, -Y, -1, +3, -4, -5, +7, -10, -11, -12, -13, -14, -15, -16, -17, -18, +20, +22, der(1)t(1;?)(q11;?), i(3q), idic(3)(3cen->p24::1q11->qter), der(7)t(7;?)(q33;?)x2, der(9)t(9;?)(p11;?)/del(9)(p13) - the large idic(3) marker and i(3q) derive from an earlier translocation, t(1;3)(q11;p24), present in a sideline - idic marker clearly derives from published translocation

Genomic sequencing and/or ARMS assay show that the cell line carries the BRAF V600E mutation (mu/wt), but no NRAS Q61 mutation

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

Print data sheet