Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established from the peripheral blood of a 7-year-old boy with acute myeloid leukemia (AML FAB M2) (in 2nd relapse after bone marrow transplantation) in 1989; cells carry the t(8;21) leading to RUNX1-RUNX1T1 (AML1-ETO) fusion gene; described in the literature to carry the KIT mutation N822. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq); part of the DSMZ "
leukemia-lymphoma 100" RNA-Seq database
Risk assessment:
The cell line was shown to exhibit reverse transcriptase (RT) activity detected by RT-ELISA. However, HIV-1 and -2 or HTLV-I and -II sequences could not be detected by PCR analysis. The cell line is thus categorized biosafety level 1.
Permissions and restrictions:
Morphology:
round cells growing singly or in small clumps in suspension;
image;
image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
start culture with a cell concentration of >1 x 106 cells/ml and cultivate initially in 24-well plates; viability may drop below 50% within the first 24 hours after thawing; in log-phase, optimal density is at about 0.5 x 106 cells/ml and a split ratio of saturated culture about 1:2 every 2-3 days is recommended; a significant amount of cell debris is always visible in the background which might be due to granula or other intracellular material
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48-72 hours
Harvest:
saturation density at about 1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 +, CD38 +, CD71 +, HLA-DR +;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypodiploid karyotype - 45<2n>X, -Y, -9, -13, -16, +3mar, t(8;21)(q22;q22), der(9)t(9;?)(p22;?), der(15)t(?9;15)((?q11;?p11) - carries both partners of 8;21 translocation associated with AML (mainly FAB M2)
Molec. Genetics:
expression of fusion gene RUNX1-RUNX1T1 (AML1-ETO) confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase positive; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
