
A-427
ACC 234
Cell line:
A-427
DSMZ no.:
ACC 234
Species:
human (Homo sapiens)
Cell type:
lung carcinoma
Origin:
established from the lung adenocarcinoma of a 52-year-old man; well characterized cell line (see Cellosaurus:Expasy) and a complex karyotype; listed in the CCLE (ref 18232)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
split confluent culture 1:3 every 4-5 days using trypsin/EDTA; seed out at ca. 2-3 x 106 cells/80 cm2; after thawing, recovery may be slow and adherence may be reduced
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 60-70 hours
Harvest:
cell harvest of about 25 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near triploid karyotype with 2% polyploidy - 67(59-72)<3n>XXY, +3, +7, -13, -14, -15, -18, -19, -20, -22, +4mar, add(1)(p11), del(1)(q11), del(3)(p11)x2, der(3)t(1;3)(q21;q11), add(4)(q31), del(6)(q21), del(7)(p11), i(10q), add(11)(q24), add(14)(p11), add(15)(p13), add(18)(q23) - 3p- deletion is characteristic of lung carcinoma - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list