EFO-21
ACC 235
Cell line:
EFO-21
DSMZ no.:
ACC 235
Species:
human (Homo sapiens)
Cell type:
ovary cystadenocarcinoma
Origin:
established from the ascitic fluid of a 56-year-old woman with dedifferentiated serous cystadenocarcinoma of the ovary in 1979
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine + 1x MEM non-essential amino acids + 1 mM sodium pyruvate
Subculture:
seed out about 1-2 x 106 cells/175 cm2; split confluent culture once a week using trypsin/EDTA; after thawing cells might grow very slowly along with a certain amount of cell debris
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 50-60 hours
Harvest:
cell harvest of about 6 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
apparently human karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list