IGR-1
ACC 236
Cell line:
IGR-1
DSMZ no.:
ACC 236
Species:
human (Homo sapiens)
Cell type:
melanoma
Origin:
established from the metastatic tumor in a groin lymph node of a 42-year-old man with malignant melanoma (primary tumor histology: NM)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
85-90% DMEM + 10-15% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells in a T75 flask; split confluent culture 1:3 to 1:5 every 3-5 days using trypsin/EDTA
Incubation:
at 37 °C with 10% CO2
Doubling time:
about 50-70 hours
Harvest:
cell harvest of about 30 x 106 cells of a T175 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded, hypertriploid karyotype with 10% polyploidy - 71(68-75)<3n>der(X), del(X), -Y, -2, +5, -8, +9, -10, -11, +5mar, add(X)(p21.3), del(X)(q22), add(1)(p36), der(6)t(6;9)(q22.3;q21.1)x2, add(8)(p21), add(9)(p13), del(9)(p21)x2, del(9)(q11), der(11)t(7;11)(q11;p11), add(13)(p12), add(17)(p12) - loss of 9p associated with melanoma
Molec. Genetics:
Genomic sequencing shows that the cell line carries the BRAF V600K mutation (mu/wt) or BRAF V600E/M (mu/mu), but no NRAS Q61 mutation
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list