PSI-2
ACC 238
Cell line:
PSI-2
DSMZ no.:
ACC 238
Species:
murine (mouse, Swiss mouse) (Mus musculus)
Cell type:
embryo - NIH-3T3 derivative (retroviral packaging line)
Origin:
subclone of the Swiss mouse embryo cell line NIH-3T3; PSI-2 cells are infected with a replication deficient MLV virus; cells can be used as "packaging cell line"; classified as risk category S1 according to the German Central Commission for Biological Safety (ZKBS)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% DMEM + 10% h.i. FBS
Subculture:
split confluent culture 1:10 to 1:20 every 2-3 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 34 hours
Harvest:
cell harvest of about 2-4 x 105 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypotetraploid karyotype - centric fragment present at 74% - ring present at 48% - 68/69(64-74)<4n> - a submetacentric marker arising by centric fusion present at 52%
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list