GAMG
ACC 242
Cell line:
GAMG
DSMZ no.:
ACC 242
Species:
human (Homo sapiens)
Cell type:
glioma
Origin:
established from the biopsy specimens from a 42-year-old woman with glioma (histologically defined as glioblastoma)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
adherent, large, spindle-like cells growing in monolayers; image
Medium:
90% DMEM + 10% h.i. FBS + 2 mM L-glutamine + 4x MEM non-essential amino acids
Subculture:
seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:4 to 1:6 every 4-6 days using trypsin/EDTA
Incubation:
at 37 °C with 10% CO2
Doubling time:
about 40-50 hours
Harvest:
cell harvest of about 10 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotriploid karyotype with 17% polyploidy - 66(61-67)<3n>XXX, -4, +5, +7, -10, -13, -19, -22, +dm x1-2, der(1)t(1;4)(q11;p11), del(3)(p14), der(10)t(3;10)(p14;q13), del(17)(p11), del(18)(p11) - additional subclonal rearrangements, add(2)(q11), add(7)(q11), i(9q) - simultaneous +7, -10, -22 and dm recurrent in gliomas
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list