Y-79
ACC 246
Cell line:
Y-79
DSMZ no.:
ACC 246
Species:
human (Homo sapiens)
Cell type:
retinoblastoma
Origin:
established from the primary tumor (right eye) of a 2-year-old girl in 1971; first RB cell line established and described to grow in soft agar and to initiate tumors in rabbits, partial deletion of the RB1 gene
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS + 3.5 g/L glucose + 2 mM L-glutamine
Subculture:
optimal split ratio at 1:2 two to three times per week; seed out at ca.0.25-0.5 x 106 cells/ml; after thawing, viability may drop below 50% temporarily
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 40 hours
Harvest:
cell harvest of about 1.0-1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 10% polyploidy - 47(45-48)<2n>XX, +mar, der(4)t(4;?6)(p15;p21.2), add(12)(q24.32), add(15)(q23), add(20)(q13) - large submetacentric marker with q-arm comprising HSR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list