OCI-AML5

ACC 247

Cell line:

OCI-AML5

DSMZ no.:

ACC 247

Species:

human (Homo sapiens)

Cell type:

acute myeloid leukemia

Origin:

established from the peripheral blood of a 77-year-old man with acute myeloid leukemia (AML M4) in relapse in 1990; the cells are described in the literature to respond to G-CSF, GM-CSF, IL-3, and FLT3 ligand; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to rapid outgrowth of independent cells

Reference(s):

14482, 15051

Biosafety level:

Permissions and restrictions:

Depositor-specific restrictions:

Transfer to third parties needs to be negotiated with the depositor.

DSMZ Cell Culture Data:

Morphology:

small, round cells growing singly or in clumps in suspension; image; image; image; image

Medium:

70% alpha-MEM (with ribo- and deoxyribonucleosides) + 20% h.i. FBS + 10% vol conditioned medium of cell line 5637 (DSM ACC 35) (or 10 ng/ml GM-CSF)

Subculture:

seed out at about 0.5 x 10⁶ cells/ml (we recommend to use 12- or 24-well plates); maintain at 0.5-1.5 x 10⁶ cells/ml; optimal split ratio of 1:2 to 1:4 every 2-4 days; viability may decrease after thawing, but cells recover quickly and viability will raise over the next few passages. CAVEAT: initial cytokine-dependent cells lines may become independent due to outgrowth of subclones; this selective outgrowth may be promoted by cytokine insufficiency and inadequate cell density or long-term cell culture. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of the cell line.

Incubation:

at 37 °C with 5% CO₂

Doubling time:

about 30-40 hours

Harvest:

saturation density at about 2.5 x 10⁶ cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in DAPI, microbiological culture, RNA hybridization, PCR assays

Immunology:

CD3-, CD4-, CD13-, CD14(+), CD15+, CD16-, CD19-, CD33+, CD34+, HLA-DR+, cyCD68+, cyCD3-; image

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human hyperdiploid karyotype - 48(44-48)<2n>XY, +1, +8, der(1)t(1;19)(p13;p13)

Viruses:

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Supplied as:

Delivery form		Prices

Growing culture (please inquire for exact delivery time)		1040,- €
Frozen culture		520,- €
DNA isolated from cell line (25 µg)		650,- €
DNA isolated from cell line (5 µg)		160,- €

see price list

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