OCI-AML5
ACC 247
Cell line:
OCI-AML5
DSMZ no.:
ACC 247
Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established from the peripheral blood of a 77-year-old man with acute myeloid leukemia (AML M4) in relapse in 1990; the cells are described in the literature to respond to G-CSF, GM-CSF, IL-3, and FLT3 ligand; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to rapid outgrowth of independent cells
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
70% alpha-MEM (with ribo- and deoxyribonucleosides) + 20% h.i. FBS + 10% vol conditioned medium of cell line 5637 (DSM ACC 35) (or 10 ng/ml GM-CSF)
Subculture:
seed out at about 0.5 x 106 cells/ml (we recommend to use 12- or 24-well plates); maintain at 0.5-1.5 x 106 cells/ml; optimal split ratio of 1:2 to 1:4 every 2-4 days; viability may decrease after thawing, but cells recover quickly and viability will raise over the next few passages. CAVEAT: initial cytokine-dependent cells lines may become independent due to outgrowth of subclones; this selective outgrowth may be promoted by
cytokine insufficiency and inadequate cell density or long-term cell culture. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of the cell line.
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 30-40 hours
Harvest:
saturation density at about 2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD4 -, CD14 -, CD15 +, CD19 -, CD33 +, cyCD68 +, HLA-DR +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype - 48(44-48)<2n>XY, +1, +8, der(1)t(1;19)(p13;p13)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list