COLO-677 - Discontinued
ACC 248
Cell line:
COLO-677 - Discontinued
DSMZ no.:
ACC 248
Species:
human (Homo sapiens)
Cell type:
multiple myeloma (cell line RPMI-8226)
Origin:
Caution: As determined by cytogenetic and STR analyses, COLO-677 is genetically identical to the human multiple myeloma cell line RPMI-8226. COLO-677 must not be used as a model for small cell lung cancer.
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
mostly adherent round cells growing in monolayers
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:4 to 1:6 every 3-4 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 40 hours
Harvest:
harvest of about 16 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis resulted in the STR profile of cell line RPMI-8226.
Cytogenetics:
human highly rearranged hypotriploid karyotype with 17% polyploidy - 62(58-62)<3n>XY, -X/Y, -2, -4, -4, -6, -8, -9, -9, -10, -13, -15, -16, +5mar - add(1)(p21), der(3)t(1;3)(q22;q24), add(4)(q32), add(5)(p11), del(7)(q32), add(11)(p14), add(13) (p11)x2, add(14)(q32), i(14q), add(17)(p12)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV +, SMRV -
Supplied as: