OMEGA-E
ACC 253
Cell line:
OMEGA-E
DSMZ no.:
ACC 253
Species:
murine (mouse) (Mus musculus)
Cell type:
NIH-3T3 (Swiss mouse embryo) derivative-retroviral packaging cell line
Origin:
derived from NIH-3T3 cells; high titer ecotropic helper-free packaging cell line; generated with separate gagpol and ecotropic env expression constructs with minimal sequence overlap and decreased sequence homology achieved by "codon wobbling"; prior to use in nude mice, cells need to be subcloned; classified as risk category S1 according to the German Central Commission for Biological Safety (ZKBS)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% DMEM + 10% NCS
Subculture:
seed out in a T75 flask and split confluent culture about 1:5 every 3-4 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 20-30 hours
Harvest:
cell harvest of about 5-8 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypertriploid karyotype - 71(63-73)<3n> - 1-2 dm (24% cells)
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list