COLO-783
ACC 257
Cell line:
COLO-783
DSMZ no.:
ACC 257
Species:
human (Homo sapiens)
Cell type:
melanoma
Origin:
established in 1990 from the right axillary lymph node of a 48-year-old woman with metastatic malignant melanoma in the right chest; no direct publication, but genome and proteome analysed; listed in the CCLE (ref 18232)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
cells grow very slowly; seed out at about 2-3 x 106 cells/80 cm2; split confluent culture 1:2 to 1:3 every 1-2 weeks using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 5-7 days
Harvest:
cell harvest of about 20 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotriploid karyotype with 20% polyloidy - 65(61-69)<3n>XXX, -4, -5, -9, -10, -12, +2mar, add(4)(p11), del(6)(p21), del(7)(p14)x2
Molec. Genetics:
Genomic sequencing and/or ARMS assay show that the cell line carries the BRAF V600E mutation (mu/wt), but no NRAS Q61 mutation
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list