RD-ES
ACC 260
Cell line:
RD-ES
DSMZ no.:
ACC 260
Species:
human (Homo sapiens)
Cell type:
Ewing's sarcoma
Origin:
established from the primary tumor of a 19-year-old man with Ewing's sarcoma of the humerus in 1984
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
85% RPMI 1640 + 15% h.i. FBS
Subculture:
seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:4 every 5-6 days using trypsin/EDTA (occasional loosely adherent clumps can be detached by tapping the flask); coating the flasks with fibronectin may improve adherence and doubling time
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 60 hours
Harvest:
cell harvest of about 10 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded, near-triploid karyotype - 69(63-72)<3n>XXY/XXYY, +1, -4, -8, +9, +12, +15, -17, +18, -22, der(9)t(9;11)(p13;q11)t(11;22)(q24;q12)x2, add(14)(p11) - carries two masked copies of 11;22 translocation associated with Ewing's sarcoma - presence of type-2 EWS-FLI1 hybrid transcripts (394 bp) confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list