LXF-289
ACC 265
Cell line:
LXF-289
DSMZ no.:
ACC 265
Species:
human (Homo sapiens)
Cell type:
lung adenocarcinoma
Origin:
established from the lung adenocarcinoma of a 63-year-old man
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% Ham's F10 + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:4 every 3 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 50 hours
Harvest:
cell harvest of about 15 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded near triploid karyotype with 4% polyploidy - 69(62-76)<3n>XY, -X/Y, -1, +6, +9, -13, -14, -18, -19, +22, +3mar, add(1)(p21), i(1q), der(2)t(1;2)(p21;p23)x2, add(4)(p11/15), i(6p), del(12)(q14), i(13q), add(14)(p11), add(15)(p11), del(17)(p11)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV +, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list