AN3-CA
ACC 267
Cell line:
AN3-CA
DSMZ no.:
ACC 267
Species:
human (Homo sapiens)
Cell type:
endometrial adenocarcinoma
Origin:
established in 1962 from the lymph node metastasis resected from a 55-year-old white woman with an endometrial adenocarcinoma (epithelial undifferentiated small cell type) presenting with acanthosis nigricans; forms heterotransplantable tumors
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% MEM (with Earle's salts) + 10% h.i. FBS + 1 mM sodium pyruvate and non-essential amino acids
Subculture:
split confluent culture 1:3 to 1:8 once or twice weekly using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/25 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 55 hours
Harvest:
cell harvest of about 10-15 x 106 cells/75 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 14% polyploidy - 49(45-50)<2n>XX, +5, +6, +7, der(3)t(1;3)(q24;p22)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list