Leibniz Institute DSMZ: Details

Cell line:

DK-MG

DSMZ no.:

ACC 277

Species:

human (Homo sapiens)

Cell type:

glioblastoma

Origin:

established from the glioblastoma multiforme (right parietal, at diagnosis) of a 67-year-old woman in 1991

Reference(s):

14689

Biosafety level:

1

Permissions and restrictions:

A

DSMZ Cell Culture Data:

Morphology:

spindle, fibroblast-like cells growing mostly adherent as monolayer; image; image

Medium:

80-90% RPMI 1640 + 10-20% h.i. FBS + 2 mM L-glutamine

Subculture:

seed out at ca. 1.0 x 10⁶ cells/80 cm²; split nearly confluent culture ca. 1:5 once a week days using trypsin/EDTA

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 70-80 hours

Harvest:

cell harvest of ca. 4-5 x 10⁶ cells/80 cm²

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in DAPI, microbiological culture, RNA hybridization, PCR assays

Immunology:

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human hypodiploid karyotype with 12% polyploidy - 44(42-44)<2n>XX, +7, -9, -10, -12, -14, +mar, del(6)(q13q25), add(9)(p2?2), der(15)t(14;15)(q12;p11), del(16)(p13), del(17)(q25), add(19)(q1?3), variable numbers of dmin detectible in most cells - resembles published karyotype

Viruses:

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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