DLD-1
ACC 278
Cell line:
DLD-1
DSMZ no.:
ACC 278
Species:
human (Homo sapiens)
Cell type:
colon adenocarcinoma
Origin:
established from a colorectal adenocarcinoma; DLD-1 is a sister cell line of the cell line HCT-15 (DSMZ ACC 357)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 every 2-3 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hours
Harvest:
cell harvest of ca. 20 x 106/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, identical to sister cell line HCT-15 (ACC 357)
Cytogenetics:
human near-diploid karyotype with tetraploid (19%) and polyploid sidelines (1.9%) - 46(43-47)<2n>X/XY, add(2)(p13/p23) - corresponds to published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list