2A1
ACC 281
Cell line:
2A1
DSMZ no.:
ACC 281
Species:
murine (mouse) (Mus musculus)
Cell type:
hybridoma (anti-chicken retina)
Origin:
established by fusion of mouse NS-1 cells with spleen from a BALB/c ByI mouse immunized with White Leghorn chicken embryonic retinal and tectal membranes; secretes 2A1 monoclonal antibody which binds epitopes involved in chicken retinal morphogenesis
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
round cells growing singly in suspension or loosely adherent
Medium:
80% RPMI 1640 + 20% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement") for the first 1-2 weeks post-thawing
Subculture:
cells are difficult to culture! maintain at ca. 0.05-0.5 x 106 cells/ml; split saturated culture 1:4 to 1:8 every 2-3 days; seed out at ca. 0.2 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 25 hours
Harvest:
maximal density at ca. 0.7 x 106 cells/ml (which should not be exceeded)
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
secretion of a IgM monoclonal antibody
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypertetraploid karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list