RPMI-2650
ACC 287
Cell line:
RPMI-2650
DSMZ no.:
ACC 287
Species:
human (Homo sapiens)
Cell type:
nasal septum squamous cell carinoma
Origin:
from the pleural effusion of a 52-year-old man with anaplastic squamous cell carcinoma of the nasal septum in 1962
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% MEM (with Earle's salts) + 10% h.i. FBS + 1x non-essential amino acids
Subculture:
split confluent culture 1:4 to 1:8 every 4-5 days using trypsin/EDTA; seed out at about 0.5-1.0 x 106 cells/25 cm2; viability may decrease directly after thawing but cells do recover rapidly
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 40-50 hours
Harvest:
cell harvest of about 0.6 x 106 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-diploid karyotype with 10% polyploidy - 45(43-49)<2n>XY, -4, -16, -18, +2mar, del(1)(p11), add(4)(p16), del(8)(q22), add(14)(p11), add(14)(q32), del(19)(p13) - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list