MOLT-16
ACC 29
Cell line:
MOLT-16
DSMZ no.:
ACC 29
Species:
human (Homo sapiens)
Cell type:
T cell leukemia
Origin:
established from the peripheral blood of a 5-year-old girl with T cell acute lymphoblastic leukemia (T-ALL) at relapse in 1984 (sister cell line MOLT-17, ACC 36)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; split 1:2 to 1:3 twice a week; a significant percentage of the cells may die within the first 24 hours after thawing; we recommend to initiate cell culture in 12- or 24-well plates in the presence of 20% FBS
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30 hours
Harvest:
saturation density at 2-2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD2 +, CD3 +, CD4 -, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta +, TCRgamma/delta -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near diploid karyotype - 46(43-47)<2n>XX, t(3;11)(p21;p13), t(8;14)(q24;q11) - t(8;14)(q24;q11) and 11p13 breakpoint are associated with T-ALL (identical to MOLT-17)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list