SF-158 - Discontinued

insect - fall armyworm (Spodoptera frugiperda) (Spodoptera frugiperda)

cloned from the parent line, IPLB-SF-21-AE, derived from pupal ovarian tissue of the fall armyworm (species Spodoptera frugiperda, family Noctuidae, order Lepidoptera); cells are susceptible to infection with MNPV and other Baculoviruses; cells can be used with Baculovirus expression vectors; the line was discontinued because it was determined by targeted sequencing of the rhabdovirus genome that it is highly likely cell line SF-9

1

A

polymorphic round cells, mostly adherent, a few cells in suspension; image; image; image

80-90% TC 100 medium + 10-20% h.i. FBS (or 80-90% Grace´s insect medium + 10-20% h.i. FBS supplemented with L-Glutamine, hydrolyzed lactalbumin and yeastolate)

split culture 1:4 to 1:5 every 5-6 day (cells grow adherent, but trypsin is not necessary as cells can be detached by tapping the flask or with a rubber scraper); seed out about 1-2 x 10⁶ cells/25 cm²

25-29 °C without CO₂ ; temperature below 23 °C may lead to reduced growth and loss of adherence

about 70 hours (at room temperature)

cell harvest of about 0.2 x 10⁶ cells/cm²

frozen with 70% medium, 20% FBS, 10% DMSO

negative in DAPI, microbiological culture, RNA hybridization, PCR assays

Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Spodoptera frugiperda species.

PCR: SMRV -

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