HPD-2NR
ACC 293
Cell line:
HPD-2NR
DSMZ no.:
ACC 293
Species:
Syrian hamster (golden hamster) (Mesocricetus auratus)
Cell type:
pancreas ductal adenocarcinoma
Origin:
established from the pancreatic ductal adenocarcinoma of a female Syrian (Golden) hamster induced by treatment with BHP (chemical carcinogen); sister cell line of HPD-1NR (DSM ACC 314); described in the literature to be homotransplantable (i.e. in other syngeneic hamsters) and to carry a mutation in exon 1 of the c-Ki-ras oncogene
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
split confluent culture 1:3 to 1:8 every 2-6 days using trypsin/EDTA; seed out at about 0.4 x 105 cells/cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 hours
Harvest:
cell harvest of ca. 1.5-2.0 x 105 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mesocricetus auratus species.
Cytogenetics:
near triploid highly rearranged karyotype with 29% polyploidy - 58-67<3n>XX, -X - hamster markers present - resembles published karyotype
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list