SW-403
ACC 294
Cell line:
SW-403
DSMZ no.:
ACC 294
Species:
human (Homo sapiens)
Cell type:
colon adenocarcinoma
Origin:
established from the adenocarcinoma (grade III) of a 51-year-old woman; cells were described in the literature to produce carcinoembryonic antigen (CEA) and to be tumorigenic in nude mice
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% DMEM + 10% h.i. FBS
Subculture:
split confluent culture 1:3 to 1:4 every 3-4 days using trypsin/EDTA; seed out at about 2.5-3.0 x 106 cells/25 cm2
Incubation:
at 37 °C with 10% CO2
Doubling time:
about 40-50 hours
Harvest:
cell harvest of about 40 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotriploid karyotype with 12% polyploidy - 62(58-70)<3n>XX/XXX, +2, +2, -8, -9, -10, -10, -12, -13, -14, -18, -19, -22, +mar, der(1)t(1;1)(p21;q22), del(4)(q21), add(5)(p15), del(6)(q24-q25), i(8q), del(9)(p21), i(9q), add(13)(p13), add(14)(p13) - carries very large isochromosome-marker, does not correspond to published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list