HT-29
ACC 299
Cell line:
HT-29
DSMZ no.:
ACC 299
Species:
human (Homo sapiens)
Cell type:
colon adenocarcinoma
Origin:
established from the primary tumor of a 44-year-old woman with colon adenocarcinoma in 1964; described in the literature to be heterotransplantable forming well-differentiated grade I tumors
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% McCoy's 5A + 10% h.i. FBS
Subculture:
seed out at about 2-3 x 106 cells into a 80 cm2 flask; split confluent culture 1:3 to 1:6 every 3-6 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 40-60 hours
Harvest:
cell harvest of about 25 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypertriploid karyotype with 17.5% polyploidy - 71(63-74)<3n>XX, -X, +1, +2, +7, -8, -9, +11, -13, -13, -14, +15, -18, +19, -21, -22, +4-6mar, del(3)(p24), del(4)(q22)x1-2, add(6)(q25), add(7)(p15), i(13q), add(19)(q13) - sideline with i(17q), t(17;19)(p10;q10) - resembles published karyotype and to that of CX-1 which is a subclone - carries very large isochromosomal ABR marker
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list