JOSK-M - Discontinued
ACC 30
Cell line:
JOSK-M - Discontinued
DSMZ no.:
ACC 30
Species:
human (Homo sapiens)
Cell type:
histiocytic lymphoma (cell line U-937)
Origin:
Caution: Cytogenetic and STR analyses established unequivocally that JOSK-M is a derivative of human histiocytic lymphoma cell line U-937.
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at 0.2-0.3 x 106 cells/ml; subculture at a 1:4 to 1:5 dilution
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
cells grow up to about 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, micobiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, HLA-DR +
Authenticity:
STR analysis resulted in the STR profile of cell line U-937.
Cytogenetics:
human hyperdiploid with 4% polyploidy - 55(51-57)<2n>XXY, +X, +3, +7, +8, +19, +20, +3mar, t(1;5;7)(p21-22;q35;p11), der(3)t(1;3)(q21;q26-27), der(6)t(6;?)(p25;?), der(10)t(10;?)(p13;?), del(11)(q22-23), der(12)t(12;?)(p13;?), der(15)t(15;?)(q24;?)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as: