Species:
human (Homo sapiens)
Cell type:
hairy cell leukemia
Origin:
spontaneously immortalized peripheral blood leukocytes from a 56-year-old white man with hairy cell leukemia (HCL) at diagnosis; cells were described in the literature to grow in soft agar, to be tumorigenic in irradiated nude mice and to be negative for the HCL-associated mutation BRAF V600E. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was detected by western blot after stimulating the cells with phorbol ester / sodium butyrate. The infection was classified as potentially lytic associated with a possible production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2.
Permissions and restrictions:
Morphology:
lymphoblastoid cells growing as single cells or in clumps, in suspension;
image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
split saturated culture 1:2 to 1:5 every 2-4 days; maintain at 0.3-0.6 x 106 cells/ml; seed out at ca. 0.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 45 hours
Harvest:
saturation density of ca. 1.0-1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa +, sm/cylambda -;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-tetraploid karyotype with 3% polyploidy - 92(89-92)<4n>XXYY, add(2)(p13->23) - differs from published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
