Leibniz Institute DSMZ: Details

Cell line:

SK-MEL-1

DSMZ no.:

ACC 303

Species:

human (Homo sapiens)

Cell type:

melanoma

Origin:

established from the lymph node metastasis (thoracic duct) of a 29-year-old man with primary melanoma in 1966; cells were described in the literature to produce pigment and to lead to pigment-producing tumors upon heterotransplantation into hamster

Reference(s):

14810

Biosafety level:

1

Permissions and restrictions:

A

DSMZ Cell Culture Data:

Morphology:

mostly round cells growing singly or in clumps (occasional giant, polynucleated cells) in suspension; image; image

Medium:

80-90% RPMI 1640 + 10-20% h.i. FBS

Subculture:

maintain between 0.1-0.3 x 10⁶ cells/ml; seed out at 0.2 x 10⁶ cells/ml; due to rapid acidification of the medium, spent culture medium should be exchanged frequently; a cell density of 0.5 x 10⁶ cells/ml should not be exceeded

Incubation:

at 37 °C with 5% CO₂

Doubling time:

about 100 hours

Harvest:

ca. 0.5-1.0 x 10⁶ cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in DAPI, microbiological culture, RNA hybridization, PCR assays

Immunology:

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human hypertriploid karyotype with 4% polyploidy - 78(72-79)<3n>XXYY, +X/Y, +2, +4, -5, +6, +7, +11, -12, -13, -13, +15, -17, +19, -21, +6mar - add(2)(p24), add(3)(q11), i(4q)x2, del(6)(q22), add(6)(q27), add(7)(q35)x2, del(9)(p23)x2, add(10)(p13), del(11)(p15), del(12)(q24), add(14)(p11), add(14)(q32), i(17q), add(17)(p12), add(19)(q13), del(?22)(q11) - carries pair of large submetacentric markers - matches published karyotype

Molec. Genetics:

Genomic sequencing and/or ARMS assay show that the cell line carries the BRAF V600E mutation (mu/wt), but no NRAS Q61 mutation

Viruses:

ELISA: reverse transcriptase negative: PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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