293
ACC 305
Cell line:
293
DSMZ no.:
ACC 305
Species:
human (Homo sapiens)
Cell type:
embryonal kidney
Origin:
established from a human primary embryonal kidney transformed by adenovirus type 5 (Ad 5); classified as risk category 1 according to the German Central Commission for Biological Safety (ZKBS) but not as GMO; cell line also known as HEK-293 (human embryonic kidney-293)
Reference(s):
Biosafety level:
1
Risk assessment:
The cell line was established by transfection of the cells with sheared DNA of adenovirus type 5. The cells contain 4 to 5 copies of the left end of the virus (12% of the viral genome) including E1a and E1b genes and one copy of the right end of the virus (10% of the genome) including the E4 gene. No active viruses are produced. No GMO.
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90-95% DMEM (with 25mM HEPES) + 5-10% h.i. FBS
Subculture:
seed out at about 2-3 x 106 cells/25 cm2; split confluent culture 1:5 to 1:6 every 2-3 days (with using trypsin/EDTA, cells can be detached by tapping the flask)
Incubation:
at 37 °C with 5-10% CO2
Doubling time:
about 24-30 hours
Harvest:
cell harvest of about 20 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-triploid karyotype with 6% polyploidy - 69(64-69)XX?X, +1, +6, -10, -13, -16, -18, -20, +21, +22, +mar, add(?X)(p22), inv(1)(p35q43), del(1)(p35), i(5p), del(6)(q11), add(8)(q24), add(13)(p13), add(17)(qter), der(?)t(5;?)(q13;?)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list