ECV-304 - Discontinued
ACC 310
Cell line:
ECV-304 - Discontinued
DSMZ no.:
ACC 310
Species:
human (Homo sapiens)
Cell type:
urinary bladder carcinoma (cell line T-24)
Origin:
STR analysis demonstrated that ECV-304 is genetically identical to the human urinary bladder carcinoma cell line T-24. ECV-304 must not be used as a model for human umbilical vein endothelial cells.
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
adherent growth as cobblestone monolayers
Medium:
80-90% Medium 199 (with HEPES buffer + Earle's salts) + 10-20% h.i. FBS
Subculture:
optimal split ratio of 1:10 to 1:20 once or twice a week using trypsin/EDTA; seed out ca. 1-2 x 104 cells/cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 2 days
Harvest:
cell harvest of max. 10 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis resulted in the STR profile of cell line T-24.
Cytogenetics:
human hypertriploid karyotype with 6.7% polyploidy - 78(75-81)<3n>XX, -X, +1, -4, +5, +5, +5, +7, -8, -9, -10, +14, -15, -15, +17, +18, +20, +20, +20, +21, +22, +4-6mar - add(1)(p32)x1-2, i(1p), der(2q;5q), der(3)t(3;11)(p25;q13), i(5p)x2, inv(7)(p14q31), add(7)(q22), del(9)(p22), add(11)(p15), der(14)t(7;14)(q21;q24), add(18)(p11)x2, add(?19) (q11) - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as: