SISO
ACC 327
Cell line:
SISO
DSMZ no.:
ACC 327
Species:
human (Homo sapiens)
Cell type:
cervix adenocarcinoma
Origin:
established from the surgically removed, chemotherapy-resistant cervix adenocarcinoma of a 67-year-old woman (clinical stage FIGO Ib)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
epithelial growth in compact colonies; cells tend to pile up after confluency; image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
split confluent culture 1:5 to 1:10 every 5-7 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/175 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35 hours
Harvest:
cell harvest of ca. 40 x 106 cells/175 cm2 (ca. 2.3 x 105 cells/cm2)
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
original contamination was eliminated with Ciprobay (ciprofloxacin), thereafter negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotriploid karyotype with 12% polyploidy - 61<3n>XXX, -1, -2, -3, -4, -6, -8, -10, -13, -14, -16, -17, -19, -22, +4mar, add(X)(q28), add(5)(p15), add(6)(p23), del(9)(q34), add(11)(q24), del(12)(q15), der(12)t(12;17)(p12;q11), add(13)(p12), add(18)(q23)x1-2 - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list