JTC-15
ACC 329
Cell line:
JTC-15
DSMZ no.:
ACC 329
Species:
rat (Rattus norvegicus)
Cell type:
hepatoma
Origin:
established in 1963 from the ascites of a rat with hepatoma induced with 4-dimethylaminoazobenzene
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% DMEM + 10% h.i. FBS
Subculture:
split confluent culture 1:5 to 1:8 every 4-5 days using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/175 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30 hours
Harvest:
cell harvest of ca. 30 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Cytogenetics:
rat hyperdiploid/hypotriploid karyotype with 33% polyploidy - 51(51-58)<2n> - secondary constriction marker present - resembles published karyotype
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list