VERO-B4
ACC 33
Cell line:
VERO-B4
DSMZ no.:
ACC 33
Species:
monkey - African green monkey (Chlorocebus aethiops (Cercopithecus aethiops))
Cell type:
kidney
Origin:
established from the kidney of a normal adult African green monkey in Japan in 1962; cells are susceptible to a number of viruses; used in virus replication studies and plaque assays, and as indicator cell line for mycoplasma testing
Reference(s):
Biosafety level:
1
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5-1.0 x 106 cells/80 cm2; split confluent culture 1:3 to 1:10 using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 25 hours
Harvest:
cell harvest of ca. 20 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Chlorocebus aethiops
Cytogenetics:
simian rearranged flat-moded hypodiploid karyotype with 12% polypoidy - 54(48-61)<2n>X - VERO-B4 markers present
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list