M1
ACC 331
Cell line:
M1
DSMZ no.:
ACC 331
Species:
murine (mouse) (Mus musculus)
Cell type:
myeloid leukemia
Origin:
bone marrow from SL mouse with spontaneous myeloid leukemia was injected into isogenic mice and the cell line was established from the ensuing hyperplastic lymph nodes 14 days later by recloning on soft agar; homotransplantable and forms colonies on soft agar (granulocytic-macrophage differentation inducible by IL-3, LPS or dexamethasone)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% mixture of MEM with Earle's salts + DMEM (at 1:1) + 10-20% h.i. FBS + 2x non-essential amino acids + 2x vitamins
Subculture:
maintain at 0.5-1.0 x 106 cells/ml; optimal split ratio of 1:2 to 1:4 every 3-5 days; seed out at 0.5-0.7 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 25-50 hours
Harvest:
saturation density at ca. 2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypodiploid karyotype with 20% polyploidy - 30(29-30)<2n>XY, 6-8 centric fusion markers present
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list