ST-2
ACC 333
Cell line:
ST-2
DSMZ no.:
ACC 333
Species:
murine (mouse) (Mus musculus)
Cell type:
stromal cells
Origin:
established from Whitlock-Witte type long-term bone marrow culture of BC8 mice
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out 0.5-1.0 x 106 cells/80 cm2; split nearly confluent culture 1:4 to 1:5 every 3-5 days using trypsin/EDTA (cell layer might detach spontaneously when cells grow too densely)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-50 hours
Harvest:
cell harvest of ca. 5 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine flat-moded hypotetraploid karyotype - 68-77<4n> - centric fusion marker present in most cells
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list