MHH-CALL-3
ACC 339
Cell line:
MHH-CALL-3
DSMZ no.:
ACC 339
Species:
human (Homo sapiens)
Cell type:
B cell precursor leukemia
Origin:
established from the bone marrow of an 11-year-old girl with pre B cell acute lymphoblastic leukemia (ALL) in 1993 (at diagnosis)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
cells may be difficult to culture; seed out at ca. 1 x 106 cells/ml; cells grow best in 24-well-plates; during the first 2-6 days proliferation rate may be rather low but the medium rarely becomes acidic (yellow), however it is advisable to change the spent medium every 2-3 days; split saturated culture 1:2; minimum density at 0.8 x 106 cells/ml, maximum density at ca. 5 x 106 cells/ml but optimal density at ca. 1-2 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 120 hours
Harvest:
cell harvest of ca. 2 x 106 cells/ml in a 24-well-plate
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD38 +, cyCD79a +, CD80 -, CD138 +, HLA-DR +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human pseudodiploid karyotype - 46(45-46)<2n>XX, del(6)(q15), der(9)t(9;9)(p21;q11), der(19)t(1;19)(q23;p13) - carries t(1;19) primary and 6q- secondary rearrangements associated with pre B-ALL - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list