JK-1
ACC 347
Cell line:
JK-1
DSMZ no.:
ACC 347
Species:
human (Homo sapiens)
Cell type:
chronic myeloid leukemia in blast crisis
Origin:
established from the biopsy material of the shoulder tumor of a 62-year-old man with chronic myeloid leukemia (CML) in erythroid blast crisis in 1987; described in the literature to produce hemoglobin; cells are described in the literature to carry t(9;22) leading to BCR-ABL1 e13-a2 (b2-a2) fusion gene
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml in 24-well-plates; maintain at 0.3-1.0 x 106 cells/ml; split 1:2 to 1:3 every 2-3 days; it is recommended to culture the cells in 24-well-plates continuously
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
maximal density of ca. 1.2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, CD71 +, CD235a +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with significant polyploidy - 48(45-48)<2n>XY, +8, +22, t(1;7)(q10;q10), der(9)t(9;22)(q34;q11), der(22)t(9;22)(q34;q11)x2 - carries two Ph - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list