KYSE-30
ACC 351
Cell line:
KYSE-30
DSMZ no.:
ACC 351
Species:
human (Homo sapiens)
Cell type:
esophageal squamous cell carcinoma
Origin:
derived from well differentiated invasive squamous cell carcinoma resected from middle intra-thoracic esophagus of a 64-year-old man prior to treatment; cell line established from tumor cells heterotransplanted into athymic mice; described in the literature to be heterotransplantable in athymic mice and to carry mutations in CDKN2A, TP53 and HRAS and amplification of cERB B, MYC and CYCLIN D1
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
45% RPMI 1640 + 45% Ham's F12 + 5-10% h.i. FBS
Subculture:
seed out at ca. 1-4 x 106 cells/175 cm2; split confluent culture 1:3 to 1:10 every 2-6 days using trypsin/EDTA (rinse 2x with dPBS before adding trypsin)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30 hours
Harvest:
cell harvest of ca. 0.7 x 105 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
original contamination was eliminated with BM-Cyclin (tiamulin & minocycline), thereafter negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
highly rearranged hypertriploid karyotype with 8% polyploidy - 66-74<3n>Xder(X), -Y, +1, +1, -5, +7, -9, -10, -15, +16, -18, -18, -19, -21, -21, -21, -22, -22, +12-15mar, t(X;2)(p11;p11), ins(1;1)(p34/36;q21q32), add(1)(q11), der(1)add(1) (p21)(q22), del(2) (q24), der(2)t(2;4)(p11;q11)t(2;4)(q37;q11), del(3)(p14), add(3)(p11), add(4)(q11)x2, der(4)t(1;4)(p11;q11), add(5)(q11), add(5)(q11), add(6)(p21), i(7q), del(7)(p14), add(7)(p14), der(8)t(7;8)(q11;p11), add(9)(p24), i(10q), del(11)(q11)x1-2, add(11)(p13), del(12) (p12.2), i(13q), add(14)(q32), add(15)(p11), add(16)(p1?), add(17)(p1?), add(19)(q13.1)x2, inc. - extreme level of rearrangement
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list